TISSUE CULTURE OF Jatropha curcas

Abstract

There is mounting interest in Jatropha species as biodiesel tree to lessen the energy crises and to generally increase income in rural areas. Tissue cultures have been used for propagation of many plant species to generate material for preservation. Jatropha curcas belongs to the genus Euphorbiaceae family which possess high amount of hydrocarbons which can be used for substitute for conventional petroleum sources. The study conducted tissue culture of Jatropha curcas from embryo.  By this method callus response of 60%, 70% and 80% were recorded from media supplemented by 1, 2 and 3mg/l 2- 4D respectively. The highest shoot induction of 1.4±0.0 was observed in supplemented medium of 1mg/LBAP +2mg/L/IBA. The study revealed an efficient and reliable plant regeneration protocol that can be potentially utilized for conservation and mass production of J. curcas to meet the demand of energy plantation as well as for the rapeutic purpose.