OPTIMIZATION OF LIPASE FROM BACTERIA ISOLATED FROM SOIL

Abstract

Lipases catalyze the hydrolysis of triacylglycerols to glycerol and fatty acids. Microbial lipases are receiving much attention because of their industrial potential. This study focuses on optimization of lipase from bacteria isolated from soil. Using pour plate method for isolation, the lipase producing isolate was identified and optimization of the effect of different parameters (pH and temperature) on the activity of lipase produced was evaluated. Bacillus subtilis was identified as the lipase producer. The optimization of pH and temperature showed slight effect on the activity of lipase. Lipase activity was observed to be maximal at optimum pH 8.0 as 1.681μmol/min and maximal at optimum temperature 50°C as 1.504μmol/min. As pH and temperature increases respectively lipase activity reduces p-value < 0.05. Bacillus subtilis from this study demonstrates remarkable lipase producing potential. Further study of the characterization and purification for industrial and environmental applications particularly toward bio treatment of oil-contaminated and polluted environment is essential.